|
KMID : 0358920040310040651
|
|
Journal of the Korean Academy of Pedodontics
2004 Volume.31 No. 4 p.651 ~ p.658
|
|
|
EXPRESSION PATTERN OF RUNX2 IN MURINE TOOTH DEVELOPMENT
|
|
Kim Tae-Wan
Ryoo Hyun-Mo
Nam Soon-Hyeun
Kim Young-Jin
Kim Hyun-Jung
|
|
|
Abstract
|
|
|
Runx2 is a transcription factor in homologous with Drosophila runt gene and it is essential for bone formation during embryogenesis and a critical gene for osteoblast differentiation and osteoblast function. Runx2-haploinsufficency causes cleidocranial dysplasia (CCD). CCD is an autosomal-dominant inherited disorder characterized by hypoplastic clevicle and delayed ossification in fontanelles and wormian bones. Dental defects are possibly shown to CCD patients : multiple supernumerary teeth, irregular and compressed permanent tooth crowns, hypoplastic and hypomineralized defects in enamel and dentin, an excess of epithelial root remnants, the absence of cellular cementum, and abnormally shaped roots. In addition, delayed eruption of the secondary dentition is a constant finding.
The aim of this study is to evaluate the role of Runx2 in the tooth development and eruption through analyzing the expression pattern of Runx2 by in situ hybridization during crown (late bell stage) and root formation of tooth, using postnatal day 1, 4, 7, 14 and 21 mice mandibular molar teeth. mRNA of Runx2-full length is expressed in dental follicle and surrounding tissue at postnatal day1 and 4. At post-natal day 7, it is expressed in ameloblasts of occlusal surface of enamel and bone area surrounding the tooth. In comparison with previous stage, at postnatal day 14, it is expressed in ameloblasts of proximal surface of enamel. At postnatal day 21 it’s expression is observed only in bone area. mRNA of Runx2-typeⅡ is not expressed At postnatal day 1 and 7. At postnatal day 14 and 21, it’s expression is observed in the bone area.
In this study, we suggest that Runx2 have a relation of ameloblasts differentiation and an important role to tooth eruption made by dental follicle during intraosseous eruption stage. Also we can confirm that Runx2 has a role to bone formation.
|
|
|
KEYWORD
|
|
|
Runx2, Dental follicle, Cleidocranial Dysplasia, Tooth Development, Tooth Eruption
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|